In this regard, xenograft mouse models represent an attractive strategy. The development of such therapies and their translation into the clinic relies on the use of animal models that mimic features of the human disease. Therefore, stage-specific and patient-specific innovative therapies are urgently needed. Altogether, fewer than 30 to 40% of patients with NB survive despite aggressive treatment combinations that involve multiagent chemotherapy, surgery, autologous bone marrow transplantation, and radiation ( 3- 5). The incidence of metastasis to various sites at diagnosis is as follows: 70.5% to bone marrow, 55.7% to bone, 30.9% to lymph nodes, 29.6% to liver, 18.2% to the cranium, 3.3% to lung, and 0.6% to the CNS ( 3). About half of all patients with NB present metastasis at the time of diagnosis ( 2). The main clinical problem in treating NB is metastasis, specifically metastatic stages III and IV according to the International Neuroblastoma Staging System ( 1, 2), because metastatic lesions are often resistant to current therapies. It is also responsible for approximately 15% of all paediatric oncology deaths ( 2). Neuroblastoma (NB) is the most common and deadly extracranial solid tumor of childhood as it accounts for 6-8% of all childhood cases of cancer ( 1). Therefore, this xenograft NB model should prove useful in testing the efficacy of new therapeutic approaches for NB. These results clearly demonstrate the higher tumorigenic and metastatic potential of NB cells in NSG mice. We also showed that cisplatin chemotherapy was able to inhibit tumor growth. In addition, tumor growth rates and organ infiltration patterns associated with injected NB cell lines correlated with the in vitro proliferation properties and genetic markers of poor prognosis in NB patients. NSG mice injected intravenously showed a profile of distant metastatis that was not observed in nude mice. Subcutaneous injection of cell lines induced tumor formation only in NSG mice and was accompanied by metastasis to the liver, adrenal glands, skull and bone marrow. To avoid charges, cancellations for timed mated animals must be received one week prior to mating date.In order to develop a relevant xenogenic animal model of neuroblastoma (NB), we compared the tumorigenicity and metastatic potential of SK-N-SH and SK-N-DZ NB cell lines in nude mice and NOD/SCID Il2rg null (NSG) mice. Therefore Envigo cannot be held responsible for actual gestation and/or exact day of littering. A variation of three to four days gestation can be expected. ** Untimed pregnant rodents will be selected from our breeding colonies on the basis of palpation or visual confirmation. * Plug guarantee only no guaranteed pregnancy. Untimed pregnant ≥ 13 days gestation (at shipping) Timed mated ≥ 13 days gestation and over (at shipping) Timed mated <13 days gestation (at shipping) If problems regarding gestational age or pregnancy are encountered, customers should immediately contact Envigo’s Customer Service Department and provide detailed information regarding the animals involved. Please be aware that late-term pregnant animals may deliver their litter prematurely while in transit, and depending on the circumstances involved, requests for credit or replacement of these animals may be declined at our sole discretion. In addition, Envigo cannot guarantee the minimum number of offspring per litter. Plug date is considered to be day zero (0) of gestation.ĭue to the natural variation in the length of gestation, the exact day of parturition cannot be guaranteed. Time mated rats and mice are determined by observation of a vaginal plug. We use an Impedance Meter for determining the stage of estrus in rats prior to breeding. Envigo uses well-established techniques to successfully produce time mated rats, mice, hamsters, and rabbits.
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